| 论文题目 | Spatio-Temporal Variation of Synechococcus Assemblages at DNA and cDNA Levels in the Tropical Estuarine and Coastal Waters |
| 论文题目（英文） | Spatio-Temporal Variation of Synechococcus Assemblages at DNA and cDNA Levels in the Tropical Estuarine and Coastal Waters |
| 作者 | 王挺;Xiaomin Xia;陈家炜;Hongbin Liuv;荆红梅 |
| 发表年度 | 2022-03-03 |
| 卷 | 13 |
| 期 | |
| 页码 | 15 |
| 期刊名称 | FRONTIERS IN MICROBIOLOGY |
| 摘要 | 聚球藻是全球海洋初级生产的主要贡献者。本研究在DNA和cDNA水平上研究了其在南海三个站位丰度和系统发育结构的时空变化。聚球藻细胞丰度在三月份最低，但在十月份两个沿海站点最高。其丰度在河口站位较高，在4月份达到峰值1.36×105细胞/ ml，这是可能是由于三亚河中氮营养盐的输入。通过定量PCR研究了四个聚球藻分支II、III、VIII和S5.3的基因和基因转录丰度，发现分支II在DNA和cDNA水平上都是最丰富的分支。高通量测序结果表明，在DNA水平上，聚球藻群落在近岸海域以SY4(在这项研究中定义的一个新的分支)、S5.2和II为主，在河口海域以淡水/ S5.2聚球藻为主，6月份达到88.61 %。季节性季风强迫和河流排放引起的盐度和营养盐浓度的变化是DNA水平上聚球藻群落时空变化的关键决定因素。相比之下，同站位、同季节样品间的高度异化，导致聚球藻群落在c DNA水平上的时空变化格局不明显。此外，共现网络揭示了聚球藻群落在cDNA水平上具有更紧密、更复杂的内部相互作用。这些差异突出了在DNA和cDNA水平上研究聚球藻组合的必要性。 |
| 摘要_英文 | Synechococcus is a major contributor to global marine primary production. Here, its spatio-temporal variations in abundance and phylogenetic structure were studied at three stations of the South China Sea at both DNA and cDNA levels. Synechococcus cell abundance was lowest in March, but highest in October at two coastal stations. Its abundance was higher at the estuarine station, which reached a peak value of 1.36 x 10(5) cells/ml in April, owing to the nitrogen nutrients discharged from the Sanya River. Gene and gene transcript abundances of four Synechococcus lineages, clades II, III, VIII, and S5.3, were studied by quantitative PCR, which showed that clade II was the most abundant lineage at both DNA and cDNA levels. High-throughput sequencing revealed that, at the DNA level, Synechococcus assemblage was dominated by clade SY4 (a novel clade defined in this study), S5.2, and clade II in the coastal waters and was dominated by freshwater/S5.2 Synechococcus, reaching a value up to 88.61% in June, in estuarine waters. Changes in salinity and nutrient concentration caused by seasonal monsoonal forcing and river discharge were the key determinants of the spatio-temporal variation in Synechococcus assemblages at the DNA level. In comparison, high dissimilation among samples at the same stations and in the same seasons leads to the imperceptible spatio-temporal variation pattern of Synechococcus assemblages at the cDNA level. Furthermore, co-occurrence networks disclosed that Synechococcus community had closer and more complex internal interactions at the cDNA level. These discrepancies highlighted the necessity to study Synechococcus assemblages at both DNA and cDNA levels. |
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